Loss of eating control and cognitive flexibility: Involvement of gut microbiota
Keywords:
Loss of eating control, obesity, food addiction, gut microbiota, cognitive flexibility, fecal microbiota transplantAbstract
Loss of eating control is a crucial factor in developing obesity, which has become a global health concern, causing important cardiovascular, metabolic, emotional, and cognitive co-morbidities. A major cognitive alteration associated with loss of eating control and obesity is the impairment of cognitive flexibility and inhibitory control. An increasing number of studies confirm that gut microbiota is a significant contributor to loss of eating control, obesity, and cognitive function. Therefore, we have investigated whether gut microbiota transfer from humans with impaired/not impaired cognitive flexibility could substantially affect this behavioral response in mice in the context of obesogenic versus standard diet. Mice were pretreated with an antibiotic cocktail and later received a gut microbiota transplant from human subjects. The transferred microbiota was maintained in mice for seven weeks. Afterward, behavioral tests were performed to evaluate different cognitive responses, locomotor activity, anxiety-like, and depression-like behaviors. Antibiotic treatment significantly impaired short-term memory in mice, as previously reported. Furthermore, mice that received microbiota from high and low cognitive flexibility subjects modified their short-term and long-term memory performance depending on the diet exposure. Slight changes were observed in the locomotor activity, primarily in the high-fat diet-fed antibiotic-treated mice, and no significant alterations were observed in anxiety-like or depressive-like behaviors. In summary, this study shows that gut microbiota is a major contributor to cognitive flexibility, which may open novel therapeutic strategies for combating loss of eating control and related metabolic co-morbidities.References
Alboni, S., Micioni Di Bonaventura, M. V., Benatti, C., Giusepponi, M. E., Brunello, N. y Cifani, C. (2017). Hypothalamic expression of inflammatory mediators in an animal model of binge eating. Behavioural Brain Research, 320, 420–430. https://doi.org/10.1016/J.BBR.2016.10.044
Arnoriaga-Rodríguez, M., Mayneris-Perxachs, J., Burokas, A., Contreras-Rodríguez, O., Blasco, G., Coll, C., Biarnés, C., Miranda-Olivos, R., Latorre, J., Moreno-Navarrete, J. M., Castells-Nobau, A., Sabater, M., Palomo-Buitrago, M. E., Puig, J., Pedraza, S., Gich, J., Pérez-Brocal, V., Ricart, W., Moya, A.,… Fernández-Real, J. M. (2020a). Obesity Impairs Short-Term and Working Memory through Gut Microbial Metabolism of Aromatic Amino Acids. Cell Metabolism, 32(4), 548-560.e7. https://doi.org/10.1016/J.CMET.2020.09.002
Arnoriaga-Rodríguez, M., Mayneris-Perxachs, J., Burokas, A., Pérez-Brocal, V., Moya, A., Portero-Otin, M., Ricart, W., Maldonado, R. y Fernández-Real, J. M. (2020b). Gut bacterial ClpB-like gene function is associated with decreased body weight and a characteristic microbiota profile. Microbiome, 8(1). https://doi.org/10.1186/S40168-020-00837-6
Arnoriaga-Rodríguez, M., Mayneris-Perxachs, J., Contreras-Rodríguez, O., Burokas, A., Ortega-Sanchez, J. A., Blasco, G., Coll, C., Biarnés, C., Castells-Nobau, A., Puig, J., Garre-Olmo, J., Ramos, R., Pedraza, S., Brugada, R., Vilanova, J. C., Serena, J., Barretina, J., Gich, J., Pérez-Brocal, V.,… Fernández-Real, J. M. (2021). Obesity-Associated Deficits in Inhibitory Control Are Phenocopied to Mice through Gut Microbiota Changes in One-Carbon and Aromatic Amino Acids Metabolic Pathways. Gut, 0, 26. https://doi.org/10.1136/gutjnl-2020-323371
Berding, K., Vlckova, K., Marx, W., Schellekens, H., Stanton, C., Clarke, G., Jacka, F., Dinan, T. G. y Cryan, J. F. (2021). Diet and the Microbiota–Gut–Brain Axis: Sowing the Seeds of Good Mental Health. Advances in Nutrition, 12(4), 1239. https://doi.org/10.1093/ADVANCES/NMAA181
Bjursell, M., Gerdin, A. K., Lelliott, C. J., Egecioglu, E., Elmgren, A., Törnell, J., Oscarsson, J. y Bohlooly-Y, M. (2008). Acutely reduced locomotor activity is a major contributor to Western diet-induced obesity in mice. American Journal of Physiology - Endocrinology and Metabolism, 294(2), 251–260. https://doi.org/10.1152/AJPENDO.00401.2007/ASSET/IMAGES/LARGE/ZH10010852180006.JPEG
Bocarsly, M. E., Fasolino, M., Kane, G. A., Lamarca, E. A., Kirschen, G. W., Karatsoreos, I. N., McEwen, B. S. y Gould, E. (2015). Obesity diminishes synaptic markers, alters Microglial morphology, and impairs cognitive function. Proceedings of the National Academy of Sciences of the United States of America, 112(51), 15731–15736. https://doi.org/10.1073/PNAS.1511593112/SUPPL_FILE/PNAS.1511593112.SAPP.PDF
Burokas, A., Martín-García, E., Espinosa-Carrasco, J., Erb, I., McDonald, J., Notredame, C., Dierssen, M. y Maldonado, R. (2018). Extinction and reinstatement of an operant responding maintained by food in different models of obesity. Addiction Biology, 23(2), 544–555. https://doi.org/10.1111/ADB.12597
Burokas, A., Martín-García, E., Gutiérrez-Cuesta, J., Rojas, S., Herance, J. R., Gispert, J. D., Serra, M. Á. y Maldonado, R. (2014). Relationships between serotonergic and cannabinoid system in depressive-like behavior: A PET study with [11C]-DASB. Journal of Neurochemistry, 130(1), 126–135. https://doi.org/10.1111/JNC.12716
Castells-Nobau, A., Puig, I., Motger-Albertí, A., de la Vega-Correa, L., Rosell-Díaz, M., Arnoriaga-Rodríguez, M., Escrichs, A., Garre-Olmo, J., Puig, J., Ramos, R., Ramió-Torrentà, L., Pérez-Brocal, V., Moya, A., Pamplona, R., Jové, M., Sol, J., Martin-Garcia, E., Martinez-Garcia, M., Deco, G.,… Mayneris-Perxachs, J. (2024). Microviridae bacteriophages influence behavioural hallmarks of food addiction via tryptophan and tyrosine signalling pathways. Nature Metabolism. https://doi.org/10.1038/S42255-024-01157-X
Cheke, L. G., Simons, J. S. y Clayton, N. S. (2016). Higher body mass index is associated with episodic memory deficits in young adults. Quarterly Journal of Experimental Psychology (2006), 69(11), 2305. https://doi.org/10.1080/17470218.2015.1099163
Chen, L., Guo, L., Feng, S., Wang, C., Cui, Z., Wang, S., Lu, Q., Chang, H., Hang, B., Snijders, A. M., Mao, J. H., Lu, Y. y Ding, D. (2023). Fecal microbiota transplantation ameliorates type 2 diabetes via metabolic remodeling of the gut microbiota in db/db mice. BMJ Open Diabetes Research & Care, 11(3), e003282. https://doi.org/10.1136/BMJDRC-2022-003282
Chen, Y., Zhou, J. y Wang, L. (2021). Role and Mechanism of Gut Microbiota in Human Disease. Frontiers in Cellular and Infection Microbiology, 11. https://doi.org/10.3389/FCIMB.2021.625913/FULL
DiSabato, D. J., Quan, N. y Godbout, J. P. (2016). Neuroinflammation: The Devil is in the Details. Journal of Neurochemistry, 139(Suppl 2), 136. https://doi.org/10.1111/JNC.13607
Domingo-Rodriguez, L., Ruiz de Azua, I., Dominguez, E., Senabre, E., Serra, I., Kummer, S., Navandar, M., Baddenhausen, S., Hofmann, C., Andero, R., Gerber, S., Navarrete, M., Dierssen, M., Lutz, B., Martín-García, E. y Maldonado, R. (2020). A specific prelimbic-nucleus accumbens pathway controls resilience versus vulnerability to food addiction. Nature Communications, 11(1), 1–16. https://doi.org/10.1038/s41467-020-14458-y
Dong, T. S., Mayer, E. A., Osadchiy, V., Chang, C., Katzka, W., Lagishetty, V., Gonzalez, K., Kalani, A., Stains, J., Jacobs, J. P., Longo, V. D. y Gupta, A. (2020). A Distinct Brain-Gut-Microbiome Profile Exists for Females with Obesity and Food Addiction. Obesity (Silver Spring, Md.), 28(8), 1477. https://doi.org/10.1002/OBY.22870
El-Sayed, A., Aleya, L. y Kamel, M. (2021). Microbiota’s role in health and diseases. Environmental Science and Pollution Research International, 28(28), 36967. https://doi.org/10.1007/S11356-021-14593-Z
Espinosa-Carrasco, J., Burokas, A., Fructuoso, M., Erb, I., Martín-García, E., Gutiérrez-Martos, M., Notredame, C., Maldonado, R. y Dierssen, M. (2018). Time-course and dynamics of obesity-related behavioral changes induced by energy-dense foods in mice. Addiction Biology, 23(2), 531–543. https://doi.org/10.1111/ADB.12595
Fumagalli, A., Castells-Nobau, A., Trivedi, D., Garre-Olmo, J., Puig, J., Ramos, R., Ramió-Torrentà, L., Pérez-Brocal, V., Moya, A., Swann, J., Martin-Garcia, E., Maldonado, R., Fernández-Real, J. M. y Mayneris-Perxachs, J. (2025). Archaea methanogens are associated with cognitive performance through the shaping of gut microbiota, butyrate and histidine metabolism. Gut Microbes, 17(1). https://doi.org/10.1080/19490976.2025.2455506
García-Blanco, A., Domingo-Rodriguez, L., Cabana-Domínguez, J., Fernández-Castillo, N., Pineda-Cirera, L., Mayneris-Perxachs, J., Burokas, A., Espinosa-Carrasco, J., Arboleya, S., Latorre, J., Stanton, C., Cormand, B., Fernández-Real, J. M., Martín-García, E. y Maldonado, R. (2022). miRNA signatures associated with vulnerability to food addiction in mice and humans. Journal of Clinical Investigation, 132(10). https://doi.org/10.1172/JCI156281
Gupta, A., Osadchiy, V. y Mayer, E. A. (2020). Brain–gut–microbiome interactions in obesity and food addiction. Nature Reviews Gastroenterology and Hepatology, 17(11), 655–672. https://doi.org/10.1038/s41575-020-0341-5
Higgs, S. (2015). Social norms and their influence on eating behaviours. Appetite, 86, 38–44. https://doi.org/10.1016/J.APPET.2014.10.021
Higgs, S. (2016). Cognitive processing of food rewards. Appetite, 104, 10–17. https://doi.org/10.1016/j.appet.2015.10.003
Higgs, S., Williamson, A. C., Rotshtein, P. y Humphreys, G. W. (2008). Sensory-Specific Satiety Is Intact in Amnesics Who Eat Multiple Meals. Psychological Science, 19(7), 623–628. https://doi.org/10.1111/J.1467-9280.2008.02132.X
Hou, K., Wu, Z. X., Chen, X. Y., Wang, J. Q., Zhang, D., Xiao, C., Zhu, D., Koya, J. B., Wei, L., Li, J. y Chen, Z. S. (2022). Microbiota in health and diseases. Signal Transduction and Targeted Therapy 2022 7:1, 7(1), 1–28. https://doi.org/10.1038/s41392-022-00974-4
Hrncir, T. (2022). Gut Microbiota Dysbiosis: Triggers, Consequences, Diagnostic and Therapeutic Options. Microorganisms, 10(3), 578. https://doi.org/10.3390/MICROORGANISMS10030578
Hussain, S. S. y Bloom, S. R. (2013). The regulation of food intake by the gut-brain axis: Implications for obesity. International Journal of Obesity (2005), 37(5), 625–633. https://doi.org/10.1038/IJO.2012.93
Kearns, R. (2024). Gut–Brain Axis and Neuroinflammation: The Role of Gut Permeability and the Kynurenine Pathway in Neurological Disorders. Cellular and Molecular Neurobiology 2024 44:1, 44(1), 1–18. https://doi.org/10.1007/S10571-024-01496-Z
Kelly, J. R., Borre, Y., O’ Brien, C., Patterson, E., El Aidy, S., Deane, J., Kennedy, P. J., Beers, S., Scott, K., Moloney, G., Hoban, A. E., Scott, L., Fitzgerald, P., Ross, P., Stanton, C., Clarke, G., Cryan, J. F. y Dinan, T. G. (2016). Transferring the blues: Depression-associated gut microbiota induces neurobehavioural changes in the rat. Journal of Psychiatric Research, 82, 109–118. https://doi.org/10.1016/j.jpsychires.2016.07.019
Kloock, S., Ziegler, C. G. y Dischinger, U. (2023). Obesity and its comorbidities, current treatment options and future perspectives: Challenging bariatric surgery? Pharmacology & Therapeutics, 251, 108549. https://doi.org/10.1016/J.PHARMTHERA.2023.108549
Liang, F., Lu, X., Deng, Z., Zhong, H. J., Zhang, W., Li, Q., Zhou, H. H., Liou, Y. L. y He, X. X. (2022). Effect of Washed Microbiota Transplantation on Patients With Dyslipidemia in South China. Frontiers in Endocrinology, 13. https://doi.org/10.3389/FENDO.2022.827107
López-Taboada, I., González-Pardo, H. y Conejo, N. M. (2020). Western Diet: Implications for Brain Function and Behavior. Frontiers in Psychology, 11, 23. https://doi.org/10.3389/FPSYG.2020.564413
Luo, S., Zhang, H., Jiang, X., Xia, Y., Tang, S., Duan, X., Sun, W., Gao, M., Chen, C., Zou, Z., Zhou, L. y Qiu, J. (2023). Antibiotics administration alleviates the high fat diet-induced obesity through altering the lipid metabolism in young mice. Lipids, 58(1), 19–32. https://doi.org/10.1002/LIPD.12361,
Magnusson, K. R., Hauck, L., Jeffrey, B. M., Elias, V., Humphrey, A., Nath, R., Perrone, A. y Bermudez, L. E. (2015). Relationships between diet-related changes in the gut microbiome and cognitive flexibility. Neuroscience, 300, 128–140. https://doi.org/10.1016/j.neuroscience.2015.05.016
Mancino, S., Burokas, A., Gutiérrez-Cuesta, J., Gutiérrez-Martos, M., Martín-García, E., Pucci, M., Falconi, A., D’Addario, C., Maccarrone, M. y Maldonado, R. (2015). Epigenetic and Proteomic Expression Changes Promoted by Eating Addictive-Like Behavior. Neuropsychopharmacology 2015 40:12, 40(12), 2788–2800. https://doi.org/10.1038/npp.2015.129
Martín-García, E., Burokas, A., Kostrzewa, E., Gieryk, A., Korostynski, M., Ziolkowska, B., Przewlocka, B., Przewlocki, R. y Maldonado, R. (2011). New operant model of reinstatement of food-seeking behavior in mice. Psychopharmacology, 215(1), 49–70. https://doi.org/10.1007/S00213-010-2110-6
Martín-García, E., Burokas, A., Martín, M., Berrendero, F., Rubí, B., Kiesselbach, C., Heyne, A., Gispert, J. D., Millán, O. y Maldonado, R. (2010). Central and peripheral consequences of the chronic blockade of CB1 cannabinoid receptor with rimonabant or taranabant. Journal of Neurochemistry, 112(5), 1338–13351. https://doi.org/10.1111/J.1471-4159.2009.06549.X
Mayneris-Perxachs, J., Arnoriaga-Rodríguez, M., Luque-Córdoba, D., Priego-Capote, F., Pérez-Brocal, V., Moya, A., Burokas, A., Maldonado, R. y Fernández-Real, J. M. (2020). Gut microbiota steroid sexual dimorphism and its impact on gonadal steroids: Influences of obesity and menopausal status. Microbiome, 8(1). https://doi.org/10.1186/s40168-020-00913-x
Mayneris-Perxachs, J., Castells-Nobau, A., Arnoriaga-Rodríguez, M., Martin, M., de la Vega-Correa, L., Zapata, C., Burokas, A., Blasco, G., Coll, C., Escrichs, A., Biarnés, C., Moreno-Navarrete, J. M., Puig, J., Garre-Olmo, J., Ramos, R., Pedraza, S., Brugada, R., Vilanova, J. C., Serena, J.,… Fernández-Real, J. M. (2022a). Microbiota alterations in proline metabolism impact depression. 34(5), 681-701.e10. https://pubmed.ncbi.nlm.nih.gov/35508109/
Mayneris-Perxachs, J., Castells-Nobau, A., Arnoriaga-Rodríguez, M., Garre-Olmo, J., Puig, J., Ramos, R., Martínez-Hernández, F., Burokas, A., Coll, C., Moreno-Navarrete, J. M., Zapata-Tona, C., Pedraza, S., Pérez-Brocal, V., Ramió-Torrentà, L., Ricart, W., Moya, A., Martínez-García, M., Maldonado, R. y Fernández-Real, J. M. (2022b). Caudovirales bacteriophages are associated with improved executive function and memory in flies, mice, and humans. Cell Host & Microbe, 30(3), 340-356.e8. https://doi.org/10.1016/J.CHOM.2022.01.013
Mayneris-Perxachs, J., Arnoriaga-Rodríguez, M., Garre-Olmo, J., Puig, J., Ramos, R., Trelis, M., Burokas, A., Coll, C., Zapata-Tona, C., Pedraza, S., Pérez-Brocal, V., Ramió, L., Ricart, W., Moya, A., Jové, M., Sol, J., Portero-Otin, M., Pamplona, R., Maldonado, R. y Fernández-Real, J. M. (2022c). Presence of Blastocystis in gut microbiota is associated with cognitive traits and decreased executive function. The ISME Journal, 16(9), 2181–2197. https://doi.org/10.1038/S41396-022-01262-3
Megur, A., Daliri, E. B. M., Baltriukienė, D. y Burokas, A. (2022). Prebiotics as a Tool for the Prevention and Treatment of Obesity and Diabetes: Classification and Ability to Modulate the Gut Microbiota. International Journal of Molecular Sciences, 23(11). https://doi.org/10.3390/IJMS23116097
Moore, C. F., Sabino, V., Koob, G. F. y Cottone, P. (2017). Pathological Overeating: Emerging Evidence for a Compulsivity Construct. Neuropsychopharmacology, 42(7), 1375–1389. https://doi.org/10.1038/npp.2016.269
Nicolas, S., Dohm-Hansen, S., Lavelle, A., Bastiaanssen, T. F. S., English, J. A., Cryan, J. F. y Nolan, Y. M. (2024). Exercise mitigates a gut microbiota-mediated reduction in adult hippocampal neurogenesis and associated behaviours in rats. Translational Psychiatry, 14(1). https://doi.org/10.1038/S41398-024-02904-0
Oliveras-Cañellas, N., Castells-Nobau, A., de la Vega-Correa, L., Latorre-Luque, J., Motger-Albertí, A., Arnoriaga-Rodriguez, M., Garre-Olmo, J., Zapata-Tona, C., Coll-Martínez, C., Ramió-Torrentà, L., Moreno-Navarrete, J. M., Puig, J., Villarroya, F., Ramos, R., Casadó-Anguera, V., Martín-García, E., Maldonado, R., Mayneris-Perxachs, J. y Fernández-Real, J. M. (2023). Adipose tissue coregulates cognitive function. Science Advances, 9(32). https://doi.org/10.1126/SCIADV.ADG4017
Penzenstadler, L., Soares, C., Karila, L. y Khazaal, Y. (2019). Systematic Review of Food Addiction as Measured with the Yale Food Addiction Scale: Implications for the Food Addiction Construct. Current Neuropharmacology, 17(6), 526. https://doi.org/10.2174/1570159X16666181108093520
Qiu, B., Liang, J. X. y Li, C. (2023). Effects of fecal microbiota transplantation in metabolic syndrome: A meta-analysis of randomized controlled trials. PLOS ONE, 18(7), e0288718. https://doi.org/10.1371/JOURNAL.PONE.0288718
Requena, T., Martínez-Cuesta, M. C. y Peláez, C. (2018). Diet and microbiota linked in health and disease. Food & Function, 9(2), 688–704. https://doi.org/10.1039/C7FO01820G
Samulėnaitė, S., García-Blanco, A., Mayneris-Perxachs, J., Domingo-Rodríguez, L., Cabana-Domínguez, J., Fernàndez-Castillo, N., Gago-García, E., Pineda-Cirera, L., Burokas, A., Espinosa-Carrasco, J., Arboleya, S., Latorre, J., Stanton, C., Hosomi, K., Kunisawa, J., Cormand, B., Fernández-Real, J. M., Maldonado, R. y Martín-García, E. (2024). Gut microbiota signatures of vulnerability to food addiction in mice and humans. Gut, gutjnl-2023-331445. https://doi.org/10.1136/gutjnl-2023-331445
Song, S., Li, Q., Jiang, Y., Liu, Y., Xu, A., Liu, X. y Chen, H. (2022). Do Overweight People Have Worse Cognitive Flexibility? Cues-Triggered Food Craving May Have a Greater Impact. Nutrients, 14(2). https://doi.org/10.3390/NU14020240
Underwood, E. L. y Thompson, L. T. (2016). A High-Fat Diet Causes Impairment in Hippocampal Memory and Sex-Dependent Alterations in Peripheral Metabolism. Neural Plasticity, 2016. https://doi.org/10.1155/2016/7385314
Vendrik, K. E. W., Ooijevaar, R. E., de Jong, P. R. C., Laman, J. D., van Oosten, B. W., van Hilten, J. J., Ducarmon, Q. R., Keller, J. J., Kuijper, E. J. y Contarino, M. F. (2020). Fecal Microbiota Transplantation in Neurological Disorders. Frontiers in Cellular and Infection Microbiology, 10, 98. https://doi.org/10.3389/FCIMB.2020.00098/FULL
Vijaya, A. K., Kuras, S., Šimoliūnas, E., Mingaila, J., Makovskytė, K., Buišas, R., Daliri, E. B. M., Meškys, R., Baltriukienė, D. y Burokas, A. (2024). Prebiotics Mitigate the Detrimental Effects of High-Fat Diet on memory, anxiety and microglia functionality in Ageing Mice. Brain, Behavior, and Immunity, 122, 167–184. https://doi.org/10.1016/J.BBI.2024.08.022
Volcko, K. L., Carroll, Q. E., Brakey, D. J. y Daniels, D. (2020). HIGH-FAT DIET ALTERS FLUID INTAKE WITHOUT REDUCING SENSITIVITY TO GLUCAGON-LIKE PEPTIDE-1 RECEPTOR AGONIST EFFECTS. Physiology & Behavior, 221, 112910. https://doi.org/10.1016/J.PHYSBEH.2020.112910
Vrieze, A., Van Nood, E., Holleman, F., Salojärvi, J., Kootte, R. S., Bartelsman, J. F. W. M., Dallinga-Thie, G. M., Ackermans, M. T., Serlie, M. J., Oozeer, R., Derrien, M., Druesne, A., Van Hylckama Vlieg, J. E. T., Bloks, V. W., Groen, A. K., Heilig, H. G. H. J., Zoetendal, E. G., Stroes, E. S., De Vos, W. M., … Nieuwdorp, M. (2012). Transfer of intestinal microbiota from lean donors increases insulin sensitivity in individuals with metabolic syndrome. Gastroenterology, 143(4). https://doi.org/10.1053/J.GASTRO.2012.06.031
World Health Organisation. Obesity and overweight. (2022). https://www.who.int/news-room/fact-sheets/detail/obesity-and-overweight
Wu, Z., Zhang, B., Chen, F., Xia, R., Zhu, D., Chen, B., Lin, A., Zheng, C., Hou, D., Li, X., Zhang, S., Chen, Y. y Hou, K. (2023). Fecal microbiota transplantation reverses insulin resistance in type 2 diabetes: A randomized, controlled, prospective study. Frontiers in Cellular and Infection Microbiology, 12, 1089991. https://doi.org/10.3389/FCIMB.2022.1089991/FULL
Yarullina, D., Novoselova, V., Alexandrova, A., Arslanova, A., Yakovleva, O., Shaidullov, I., Nikolaev, Y., El-Registan, G., Kudrin, V. y Sitdikova, G. (2024). Probiotic Lactobacilli Ameliorate Antibiotic-Induced Cognitive and Behavioral Impairments in Mice. Microbiology Research 2024, Vol. 15, Pages 1471-1485, 15(3), 1471–1485. https://doi.org/10.3390/MICROBIOLRES15030099
Yokoyama, Y., Nakamura, T. J., Yoshimoto, K., Ijyuin, H., Tachikawa, N., Oda, H., Shiraishi, R., Shinohara, K., Kumadaki, K., Honda, S., Nakamura, A., Kitamura, N., Tsubota, K. y Watanabe, M. (2020). A high-salt/high fat diet alters circadian locomotor activity and glucocorticoid synthesis in mice. PloS One, 15(5). https://doi.org/10.1371/JOURNAL.PONE.0233386
